Dr Cacho's research focuses on Renaissance and Baroque cultures and Spanish American colonial literature. His scholarship has been concerned with literary genres such as burlesque and epic poetry, and the works of Francisco de Quevedo.

It also treats aspects related to the transmission of culture in the early modern period, including interdisciplinary approaches such as theory of painting and the art of memory. These last are studied particularly in his monograph La esfera del ingenio: las silvas de Quevedo y la tradición europea (2012).

The relationship between art and literature is another area of his research, with a particular interest in painters such as Diego Velázquez and Juan de Valdés Leal. He also works on colonial poetry, especially on the emergence of literary communities in the New World. He has recently co-edited a volume on this subject The Rise of Spanish American Poetry, 1500-1700: Literary and Cultural Transmission in the New World (2019).